diff --git a/services/auth/basic.go b/services/auth/basic.go
index 90bd642370..1f6c3a442d 100644
--- a/services/auth/basic.go
+++ b/services/auth/basic.go
@@ -5,6 +5,7 @@
 package auth
 
 import (
+	"errors"
 	"net/http"
 	"strings"
 
@@ -141,6 +142,15 @@ func (b *Basic) Verify(req *http.Request, w http.ResponseWriter, store DataStore
 	}
 
 	if skipper, ok := source.Cfg.(LocalTwoFASkipper); !ok || !skipper.IsSkipLocalTwoFA() {
+		// Check if the user has webAuthn registration
+		hasWebAuthn, err := auth_model.HasWebAuthnRegistrationsByUID(req.Context(), u.ID)
+		if err != nil {
+			return nil, err
+		}
+		if hasWebAuthn {
+			return nil, errors.New("Basic authorization is not allowed while webAuthn enrolled")
+		}
+
 		if err := validateTOTP(req, u); err != nil {
 			return nil, err
 		}
diff --git a/tests/integration/api_twofa_test.go b/tests/integration/api_twofa_test.go
index aad806b6dc..18e6fa91b7 100644
--- a/tests/integration/api_twofa_test.go
+++ b/tests/integration/api_twofa_test.go
@@ -53,3 +53,56 @@ func TestAPITwoFactor(t *testing.T) {
 	req.Header.Set("X-Gitea-OTP", passcode)
 	MakeRequest(t, req, http.StatusOK)
 }
+
+func TestBasicAuthWithWebAuthn(t *testing.T) {
+	defer tests.PrepareTestEnv(t)()
+
+	// user1 has no webauthn enrolled, he can request API with basic auth
+	user1 := unittest.AssertExistsAndLoadBean(t, &user_model.User{ID: 1})
+	unittest.AssertNotExistsBean(t, &auth_model.WebAuthnCredential{UserID: user1.ID})
+	req := NewRequest(t, "GET", "/api/v1/user")
+	req.SetBasicAuth(user1.Name, "password")
+	MakeRequest(t, req, http.StatusOK)
+
+	// user1 has no webauthn enrolled, he can request git protocol with basic auth
+	req = NewRequest(t, "GET", "/user2/repo1/info/refs")
+	req.SetBasicAuth(user1.Name, "password")
+	MakeRequest(t, req, http.StatusOK)
+
+	// user1 has no webauthn enrolled, he can request container package with basic auth
+	req = NewRequest(t, "GET", "/v2/token")
+	req.SetBasicAuth(user1.Name, "password")
+	resp := MakeRequest(t, req, http.StatusOK)
+
+	type tokenResponse struct {
+		Token string `json:"token"`
+	}
+	var tokenParsed tokenResponse
+	DecodeJSON(t, resp, &tokenParsed)
+	assert.NotEmpty(t, tokenParsed.Token)
+
+	// user32 has webauthn enrolled, he can't request API with basic auth
+	user32 := unittest.AssertExistsAndLoadBean(t, &user_model.User{ID: 32})
+	unittest.AssertExistsAndLoadBean(t, &auth_model.WebAuthnCredential{UserID: user32.ID})
+
+	req = NewRequest(t, "GET", "/api/v1/user")
+	req.SetBasicAuth(user32.Name, "notpassword")
+	resp = MakeRequest(t, req, http.StatusUnauthorized)
+
+	type userResponse struct {
+		Message string `json:"message"`
+	}
+	var userParsed userResponse
+	DecodeJSON(t, resp, &userParsed)
+	assert.EqualValues(t, "Basic authorization is not allowed while webAuthn enrolled", userParsed.Message)
+
+	// user32 has webauthn enrolled, he can't request git protocol with basic auth
+	req = NewRequest(t, "GET", "/user2/repo1/info/refs")
+	req.SetBasicAuth(user32.Name, "notpassword")
+	MakeRequest(t, req, http.StatusUnauthorized)
+
+	// user32 has webauthn enrolled, he can't request container package with basic auth
+	req = NewRequest(t, "GET", "/v2/token")
+	req.SetBasicAuth(user1.Name, "notpassword")
+	MakeRequest(t, req, http.StatusUnauthorized)
+}