diff --git a/synapse/app/homeserver.py b/synapse/app/homeserver.py
index 29ca720d5e..afb46d2e23 100755
--- a/synapse/app/homeserver.py
+++ b/synapse/app/homeserver.py
@@ -38,8 +38,7 @@ from synapse.http.server_key_resource import LocalKey
 from synapse.http.matrixfederationclient import MatrixFederationHttpClient
 from synapse.api.urls import (
     CLIENT_PREFIX, FEDERATION_PREFIX, WEB_CLIENT_PREFIX, CONTENT_REPO_PREFIX,
-    SERVER_KEY_PREFIX, MEDIA_PREFIX, CLIENT_V2_ALPHA_PREFIX, APP_SERVICE_PREFIX,
-    STATIC_PREFIX
+    SERVER_KEY_PREFIX, MEDIA_PREFIX, CLIENT_V2_ALPHA_PREFIX, STATIC_PREFIX
 )
 from synapse.config.homeserver import HomeServerConfig
 from synapse.crypto import context_factory